Advanced IVF

Advanced IVF procedures

Nowadays, due to the advancements in medical science, advanced IVF treatments are available. These include complicated and hyper advanced procedures which seem to be outworldly and fantastic. All of these best IVF treatments are now available in Chandigarh at Jindal IVF.

  • Intracytoplasmic sperm injection (ICSI)
  • ICSI with Testicular sperm (TESA/TESE/Micro TESE/PESA)
  • IVF with OD
  • Assisted Hatching
  • Blastocyst Culture and Transfer
  • Surrogacy
  • Fertility preservation
  • Recurrent implantation failure
  • Embryoscope

Intracytoplasmic Sperm Injection (ICSI)

Just like the IVF procedure explained in the previous section, ICSI is also used for the treatment of infertility. It is an advanced type of IVF procedure in which the sperm is directly injected into the female egg with the help of a special machine known as a micro-manipulator.

ICSI with Testicular sperm (TESA/TESE/Micro TESE/PESA)

Just like the IVF procedure explained in the previous section, ICSI is also used for the treatment of infertility. It is an advanced type of IVF procedure in which the sperm is directly injected into the female egg with the help of a special machine known as a micro-manipulator.
Sometimes one needs to take sperms directly from the testes. When ICSI is done with sperms taken directly from testis it is called ICSI with testicular sperm. TESA/TESE/Micro TESE/PESA are various techniques for obtaining sperms from testis . PESA and micro TESE are the most commonly performed techniques.

  • TESA –testicular sperm aspiration
  • TESE – testicular sperm extraction
  • Micro-TESE – retrieval of best sperms from healthy tubules of testes under operating microscope
  • PESA –percutaneous epididymal sperm aspiration

IVF with Ovum Donation (IVF-OD)

This is an advanced IVF procedure where eggs are taken from another woman who donates her eggs for the procedure. It is done in females who are advanced in age or who have some problem which precludes them from using their own eggs.

Assisted Hatching

This is also an advanced IVF procedure. Normally, there is a protective shell outside the egg and embryo. The embryo comes out of it before implantation. This process is called hatching.
In some there is a difficulty in this process and hatching does not occur normally. So help is given by making a hole in the wall of the embryo by extremely sophisticated instruments to avoid any harm. This is called assisted hatching.

Blastocyst Culture and Transfer

Blastocyst Culture and Transfer is an extremely advanced type of IVF which is not possible to do everywhere. An IVF centre which is doing regular blastocyst culture and transfer is held to be extremely advanced.
Usually, after an IVF procedure, the developing embryo is transferred back in to the uterus by day 2-3 after the procedure. However, it is possible to keep the embryos in the lab for 4-6 days after fertilization before being placed back in womb. The embryo gains many cells as a result of being developed for so many days and are much more advanced than the ones developed for just three days. These Embryos are called blastocysts.
These blastocysts are much more likely to produce fruitful results. This enables embryologists to select from the more advanced embryos with better potential for implantation.
The biggest advantage of Blastocyst Culture and Transfer method is that it significantly reduces the risk of multiple pregnancies. Also, the pregnancy and implantation success rate is higher in this technique because it is easy to determine the robust embryos after developing for 4-6 days hence only the competent embryo are transferred to uterus.
We are routinely doing this technique and ours is the most advanced centre in the region.


Sometimes it is not possible for a woman to carry a pregnancy because of multiple reasons. Some of these reasons include those like removal of the uterus for some cause, badly scarred uterus because of infection or surgery, absence of uterus by birth, serious medical conditions, etc. In these situations surrogacy is employed.
When embryos are implanted in another women’s uterus (womb) then it is called surrogacy. The baby grows for nine months in the surrogate’s uterus but it is genetically linked to the couple who commissioned the surrogate.
Surrogacy is permitted under Indian Law with special safeguards to prevent any misdoing or exploitation and the child born has all the privileges and obligations as a child born in the natural way.

Fertility Preservation

Cancer is increasing globally and in India, especially in the young population. The treatment given for cancer, including chemotherapy and radiotherapy, despite preserving the life of the patient may have unintended side effects, in the form of destruction of the reproductive cells of the body. Young people, who have their whole life ahead of them, may be devastated by this. Treatments given in other diseases such as autoimmune disorders, rheumatoid arthritis, etc. may also lead to the same result.
Fertility preservation in males who have reached puberty is easy as it requires semen cryopreservation. 2 or 3 samples may be preserved before starting chemo or radiotherapy.
For females it is more difficult than in males and varies according to situation. Egg retrieval can be done in unmarried women who have attained puberty after giving hormonal injections. Eggs thus obtained can be cryopreserved for future use.
Married women who have not completed their families – an IVF cycle is performed for these patients, where ovaries are stimulated by hormonal injections to retrieve eggs. These eggs are fertilized with the husband’s sperm. Embryos thus formed are cryopreserved till the patient completes chemo or radiotherapy after which these can be implanted in the woman’s uterus.
Preservation in children is difficult and still under research.
No other centre is currently offering these services in the region.

Recurrent Implantation Failure Clinic

This type of advanced technique is meant for patients who have had multiple IVF failures. We receive a lot of referral cases of multi-failure IVF cases. These patients are then thoroughly investigated to find out the cause of IVF failure.
Treatment is directed towards correcting the underlying cause and treating it appropriately. This may include open surgery, laparoscopicsurgery, immunotherapy, etc. We serve as a tertiary level referral centre for these difficult to treat patients.


Embryoscope is advanced equipment for the purpose of embryo culture. It allows for continuous culture of embryos without any interference from outside so that embryos may grow under ideal conditions. It has-

  • Tri-gas Incubator with advanced temperature control
  • Build-in microscope for acquisition of time-lapse images
  • 6 patients with 12 embryos each = 72 samples
  • Automatic startup and un-attended operation
  • External workstation for monitoring, evaluation and image analysis


  • Reduced handling of embryos as observations made from inside incubator
  • Direct heat transfer allows for uniform distribution
  • Active carbon and HEPA filter
  • UV disinfection of circulated Air
  • Tri-gas mixer for optimal growth environment
  • Time-lapse imaging allows for better choice between embryos
  • Embryosare undisturbed and can be assessed without opening the incubatoragain and again

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